Title: Design of Portable Multicolor LED-Based Optical System for the Photobiomodulation Therapy on Wound Healing Process

Abstract: Photobiomodulation is a practical and noninvasive treatment that triggers cell proliferation, cell differentiation, wound healing, new tissue formation, inflammation and pain reduction with low-level light therapy. Light-emitting diodes (LEDs) are energy-saving, affordable and safe alternatives to laser devices which are recently preferred in photobiomodulation. Although the wavelengths between 600-700 nm are most preferred ones, there is a lack of practical optical systems which study this mechanism in vitro with different wavelengths simultaneously. In this study, a portable and remotely controlled multicolor LED-based system was designed and tested on the wound healing process of human keratinocytes by irradiating the cells homogenously with 3 different wavelengths (460-475 nm as blue, 515-535 nm as green, and 585-595 nm as orange) on different experimental groups at the same time. Its proliferative and wound healing effect was evaluated with cell viability (MTT) analysis and cell migration (scratch) assay, respectively. It was observed that orange-LEDs were designated as the most triggering wavelength in terms of cell proliferation. Also, it was revealed with this device that different wavelengths can reach the intended accelerated wound healing process, so this optical system will be an advantageous design for future practical photobiomodulation studies in vitro.

Keywords: photobiomodulation; blue LED; green LED; orange LED; wound healing

Başlık: Yara İyileştirme Sürecinde Fotobiyomodülasyon Terapisi için Çok Renkli LED Tabanlı Optik Sistem Tasarımı

Özet: Fotobiyomodülasyon, düşük seviyeli ışık tedavisi ile hücre çoğalmasını, hücre farklılaşmasını, yara iyileşmesini, yeni doku oluşumunu, iltihaplanmayı ve ağrı azalmasını sağlayan pratik ve girişimsel olmayan bir tedavidir. Işık yayan diyotlar (LED'ler), son zamanlarda fotobiyomodülasyonda sıklıkla tercih edilen lazer cihazlarına kıyasla enerji tasarrufu sağlayan, ekonomik ve güvenli alternatiflerdir. 600-700 nm arasındaki dalga boyları en çok tercih edilen dalga boyları olsa da, aynı anda farklı dalga boylarında bu mekanizmayı in vitro olarak inceleyen pratik optik sistem eksikliği söz konusudur. Bu çalışmada, taşınabilir ve uzaktan kontrollü çok renkli LED tabanlı bir optik sistem tasarlanmış ve bu sistem ile 3 farklı dalga boyunun (460-475 nm mavi, 515-535 nm yeşil, ve turuncu 585-595 nm) aynı anda hücreler üzerindeki proliferatif ve yara iyileştirici etkisi, sırasıyla hücre canlılığı (MTT) analizi ve hücre göçü (scratch) analizi ile değerlendirilmiştir. Turuncu-LED'lerin hücre proliferasyonu açısından en tetikleyici dalga boyu olduğu görülmüştür. Ayrıca bu cihazla, farklı dalga boylarının amaçlanan hızlandırılmış yara iyileşme sürecini tetikleyebileceği ortaya konulmuştur. Bu nedenle bu optik sistem, in vitro olarak gelecekteki pratik fotobiyomodülasyon çalışmaları için avantajlı bir prototip tasarımı olmuştur.

Anahtar kelimeler: fotobiyomodülasyon; mavi LED; yeşil LED; turuncu LED; yara iyileşmesi

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